Reporter
Part:BBa_K1486048:Design
Designed by: EPFL iGem team 2014 Group: iGEM14_EPF_Lausanne (2014-10-07)
CpxR reporter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 636
Illegal AgeI site found at 748 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The regulating sequence of of the RFP coding sequence is the on that is supposed to be in the BBa_K339007 but is not. The RFP has a start- and a stopcodon.
Source
The basis of this part is the BBa_K339007 which had just the RFP sequence. We inserted the non coding sequence that Calgary claims to be in the BBa_K339007 by site directed mutagenesis.