Reporter

Part:BBa_K1486048:Design

Designed by: EPFL iGem team 2014   Group: iGEM14_EPF_Lausanne   (2014-10-07)


CpxR reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 636
    Illegal AgeI site found at 748
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The regulating sequence of of the RFP coding sequence is the on that is supposed to be in the BBa_K339007 but is not. The RFP has a start- and a stopcodon.

Source

The basis of this part is the BBa_K339007 which had just the RFP sequence. We inserted the non coding sequence that Calgary claims to be in the BBa_K339007 by site directed mutagenesis.

References